New luminescent lanthanide chelates containing thiol-, amine-, and click-reactive groups in antenna-fluorophore
moieties were synthesized. The chelates include diethylenetriaminepentaacetic acid (DTPA) coupled to two types of
chromophores: 7-amino-4-trifluoromethyl-2(1H) quinolinone, and 7-amino-4-trifluoromethyl-2-alkoxyquinoline. The
synthesized compounds were characterized using NMR, light absorption, steady-state and time-resolved fluorescent
spectroscopy. Some of the compounds displayed high brightness with Tb3+, Eu3+, and Dy3+. Obtained reactive lanthanide
chelates can be easily attached to biological molecules. The probes demonstrated high performance in molecular beaconbased
DNA hybridization assays (sub-pico molar detection limit), in bacterial proteome labeling, and in live cell
imaging.
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