Improved fabrication of zero-mode waveguides for monitoring specific molecular dynamics in living cells

Chia-Fen Hsieh; Shui-Chin Lai; Chih-Ting Chen; Yii-Lih Lin; Kuo-Tang Liao; Lesser-Rojas Leonardo; K. K. Sriram; Shengqin Wang; Po-Chieh Chiang; Chia-Fu Chou

doi:10.1117/12.909797

2 February 2012 Improved fabrication of zero-mode waveguides for monitoring specific molecular dynamics in living cells

Chia-Fen Hsieh, Shui-Chin Lai, Chih-Ting Chen, Yii-Lih Lin, Kuo-Tang Liao, Lesser-Rojas Leonardo, K. K. Sriram, Shengqin Wang, Po-Chieh Chiang, Chia-Fu Chou

Author Affiliations +

Proceedings Volume 8231, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII; 82310W (2012) https://doi.org/10.1117/12.909797
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Zero-mode waveguides (ZMWs) are optical nanostructures to confine fluorescent excitation within sub-diffraction volumes and are commonly used for single-molecule analysis. However, the conventional ZMWs with aluminum film on fused silica have limitations on living cell studies. The same surfaces composed of hydroxyl group inside and outside each ZMW restrict specific surface functionalization. The sharp-cylinder shaped and rough edge of each ZMW produces a steric interference of molecular dynamics on cell membrane. In this study, selectively surface functionalization inside and outside of each ZMW was achieved with tri-metal-layer film on fused silica. Moreover, bowl-shaped and smooth edge of each ZMW was manufactured in large area. The improvement of ZMWs provides a broad way for monitoring molecular dynamics in living cells.

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Chia-Fen Hsieh, Shui-Chin Lai, Chih-Ting Chen, Yii-Lih Lin, Kuo-Tang Liao, Lesser-Rojas Leonardo, K. K. Sriram, Shengqin Wang, Po-Chieh Chiang, and Chia-Fu Chou "Improved fabrication of zero-mode waveguides for monitoring specific molecular dynamics in living cells", Proc. SPIE 8231, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 82310W (2 February 2012); https://doi.org/10.1117/12.909797

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