Comparison of analysis methods for fluorescence lifetime imaging

Tavis Hall; Dustin A. Dorroh; S. Elizabeth Robertson; D. T. Schaafsma

doi:10.1117/12.909143

2 February 2012 Comparison of analysis methods for fluorescence lifetime imaging

Tavis Hall, Dustin A. Dorroh, S. Elizabeth Robertson, D. T. Schaafsma

Proceedings Volume 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX; 82271Q (2012) https://doi.org/10.1117/12.909143
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We compare various time-gated and discrete-time methods for extracting the decay constant from an exponential signal, typical of fluorescence lifetime (FLIM) instruments, as well as a host of other physical phenomena. Analytical methods are evaluated for accuracy and precision as well as for computational and sampling efficiency.

Citation Download Citation

Tavis Hall, Dustin A. Dorroh, S. Elizabeth Robertson, and D. T. Schaafsma "Comparison of analysis methods for fluorescence lifetime imaging", Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82271Q (2 February 2012); https://doi.org/10.1117/12.909143

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