High-speed spectral tuning CARS microscopy using AOTF laser

Mamoru Hashimoto; Junichi Iwatsuka; Hirohiko Niioka; Tsutomu Araki

doi:10.1117/12.909507

9 February 2012 High-speed spectral tuning CARS microscopy using AOTF laser

Mamoru Hashimoto, Junichi Iwatsuka, Hirohiko Niioka, Tsutomu Araki

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 82261N (2012) https://doi.org/10.1117/12.909507
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We have developed a high speed spectral tuning CARS microscopy system using a mode-locked Ti:Sapphire laser with an acousto-optic tunable filter (AOTF) in the cavity. Since the wavelength of the laser is tunable with the applied radio frequency to the AOTF, the wavelength is electrically tunable.The pulse duration of the laser is about 10 ps, tunable range is 800 nm to 930 nm, and the tuning speed is ms order. The laser is synchronized with another mode-locked Ti:Sapphire laser laser our own method using a balance cross-correlator and phase lock loop technique. The synchronized lasers are used for light source of multi-focus CARS microscopy system using a microlens array scanner, and the hyperspectral imaging of adipocyte cells is demonstrated.

Citation Download Citation

Mamoru Hashimoto, Junichi Iwatsuka, Hirohiko Niioka, and Tsutomu Araki "High-speed spectral tuning CARS microscopy using AOTF laser", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82261N (9 February 2012); https://doi.org/10.1117/12.909507

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available