Immobilization of enzyme (DAAO) on hybrid nanoporous MCF, SBA-15, and MCM-41 materials

Tien Q. Phi; Hy G. Le; Tuan A. Vu; Thao T. H. Phan; Huyen T. Pham; Canh Duc Dao; Phuong T. Dang

doi:10.1117/12.903739

23 December 2011 Immobilization of enzyme (DAAO) on hybrid nanoporous MCF, SBA-15, and MCM-41 materials

Tien Q. Phi, Hy G. Le, Tuan A. Vu, Thao T. H. Phan, Huyen T. Pham, Canh Duc Dao, Phuong T. Dang

Proceedings Volume 8204, Smart Nano-Micro Materials and Devices; 82043I (2011) https://doi.org/10.1117/12.903739
Event: SPIE Smart Nano + Micro Materials and Devices, 2011, Melbourne, Australia

Abstract

Hybrid nanoporous MCF, SBA-15 and MCM-41 materials were synthesized via hydrothermal treatment and functionalized with 3-aminopropyltriethoxysilane (APTES) via post-synthesis grafting and sequently activated by glutardialdehyde and then were used to immobilize D-amino acid oxidase (DAAO). The amino-functionalized materials were characterized by various techniques: XRD, IR and N₂ adsorption-desorption (BET). From characterization results, it indicated that these materials still maintained their structure after functionalization. The data IR and TGA-DTA analysis demonstrated the incorpotation of amine functional groups on the surface of APTES-functionalized samples. The DAAO immobilized on functionalized materials exhibited higher catalytic activity and stability for conversion of cephalosporin C (CPC) compare to those of non-functionalized one. Further more, the catalytic activity as well as stability of enzyme decreased in order MCF > SBA-15 > MCM-41 with the decrease of their pore size.

Citation Download Citation

Tien Q. Phi, Hy G. Le, Tuan A. Vu, Thao T. H. Phan, Huyen T. Pham, Canh Duc Dao, and Phuong T. Dang "Immobilization of enzyme (DAAO) on hybrid nanoporous MCF, SBA-15, and MCM-41 materials", Proc. SPIE 8204, Smart Nano-Micro Materials and Devices, 82043I (23 December 2011); https://doi.org/10.1117/12.903739

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available