Application of oblique plane microscopy to high speed live cell imaging

Sunil Kumar; Dean Wilding; Markus B. Sikkel; Alexander R. Lyon; Ken T. MacLeod; Chris Dunsby

doi:10.1117/12.889841

15 June 2011 Application of oblique plane microscopy to high speed live cell imaging

Sunil Kumar, Dean Wilding, Markus B. Sikkel, Alexander R. Lyon, Ken T. MacLeod, Chris Dunsby

Proceedings Volume 8086, Advanced Microscopy Techniques II; 80860V (2011) https://doi.org/10.1117/12.889841
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

Oblique Plane Microscopy (OPM) is a light sheet microscopy technique that combines oblique illumination with correction optics that tilt the focal plane of the collection system. OPM can be used to image conventionally mounted specimens on coverslips or tissue culture dishes and has low out-of-plane photobleaching and phototoxicity. No moving parts are required to achieve an optically sectioned image and so high speed optically sectioned imaging is possible. We present high speed 2D and 3D optically sectioned OPM imaging of live cells using a high NA water immersion lens.

Citation Download Citation

Sunil Kumar, Dean Wilding, Markus B. Sikkel, Alexander R. Lyon, Ken T. MacLeod, and Chris Dunsby "Application of oblique plane microscopy to high speed live cell imaging", Proc. SPIE 8086, Advanced Microscopy Techniques II, 80860V (15 June 2011); https://doi.org/10.1117/12.889841

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