Adaptive optics in wide-field microscopy

Peter Kner; Zvi Kam; David Agard; John Sedat

doi:10.1117/12.876500

14 February 2011 Adaptive optics in wide-field microscopy

Peter Kner, Zvi Kam, David Agard, John Sedat

Proceedings Volume 7931, MEMS Adaptive Optics V; 79310K (2011) https://doi.org/10.1117/12.876500
Event: SPIE MOEMS-MEMS, 2011, San Francisco, California, United States

Abstract

Three-dimensional live imaging in cell biology is hindered by optical aberrations which degrade the resolution and signal-to-noise ratio as the focal plane is moved deeper into the sample. The solution to this problem is to use adaptive optics to correct the aberrations. In this paper, we discuss our work on applying adaptive optics to wide-field fluorescence microscopy. We demonstrate correction of depth-aberrations and focusing using a deformable mirror in open-loop operation. We then discuss the use of phase retrieval and phase diversity in adaptive optics.

Citation Download Citation

Peter Kner, Zvi Kam, David Agard, and John Sedat "Adaptive optics in wide-field microscopy", Proc. SPIE 7931, MEMS Adaptive Optics V, 79310K (14 February 2011); https://doi.org/10.1117/12.876500

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