Single-cell imaging detection of nanobarcoded nanoparticle biodistributions in tissues for nanomedicine

Trisha Eustaquio; Christy L. Cooper; James F. Leary

doi:10.1117/12.874290

11 February 2011 Single-cell imaging detection of nanobarcoded nanoparticle biodistributions in tissues for nanomedicine

Trisha Eustaquio, Christy L. Cooper, James F. Leary

Author Affiliations +

Proceedings Volume 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III; 79100O (2011) https://doi.org/10.1117/12.874290
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

In nanomedicine, biodistribution studies are critical to evaluate the safety and efficacy of nanoparticles. Currently, extensive biodistribution studies are hampered by the limitations of bulk tissue and single-cell imaging techniques. To ameliorate these limitations, we have developed a novel method for single nanoparticle detection that incorporates a conjugated oligonucleotide as a "nanobarcode" for detection via in situ PCR. This strategy magnifies the detection signal from single nanoparticles, facilitating rapid evaluation of nanoparticle uptake by cell type over larger areas. The nanobarcoding method can enable precise analysis of nanoparticle biodistributions and expedite translation of these nanoparticles to the clinic.

Citation Download Citation

Trisha Eustaquio, Christy L. Cooper, and James F. Leary "Single-cell imaging detection of nanobarcoded nanoparticle biodistributions in tissues for nanomedicine", Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79100O (11 February 2011); https://doi.org/10.1117/12.874290

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