Automated optical cell detection, sorting, and temperature measurements

Joel Kindt; Mujahid Naqbi; Torsten Kiljan; Wesley Fuller; Weina Wang; David W. Kisker; Kevin L. Lear

doi:10.1117/12.875608

22 February 2011 Automated optical cell detection, sorting, and temperature measurements

Joel Kindt, Mujahid Naqbi, Torsten Kiljan, Wesley Fuller, Weina Wang, David W. Kisker, Kevin L. Lear

Proceedings Volume 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX; 790222 (2011) https://doi.org/10.1117/12.875608
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

An automated cell detection and sorting system was developed, combining both the optofluidic intracavity spectroscopy (OFIS) technique and dielectrophoresis (DEP). The OFIS method utilizes a microfluidic channel as a Fabry-Perot cavity to produce characteristic transmission spectra of individual cells. The concept behind optical detection is that a decrease in spectral intensity beyond a threshold indicates that a cell is present. Upon detection, an RF voltage is automatically applied to electrodes, trapping the cell with DEP forces. The system then sorts the cell into one of two microfluidic channels based on resulting optical analysis. A further advantage is that RF joule heating can be measured from known dn/dT values of the medium, which is useful for investigating cell viability issues.

Citation Download Citation

Joel Kindt, Mujahid Naqbi, Torsten Kiljan, Wesley Fuller, Weina Wang, David W. Kisker, and Kevin L. Lear "Automated optical cell detection, sorting, and temperature measurements", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 790222 (22 February 2011); https://doi.org/10.1117/12.875608

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