Long-term time-lapse multimodal microscopy for tracking cell dynamics in live tissue

Benedikt W. Graf; Maria C. Valero; Eric J. Chaney; Marina Marjanovic; Marni D. Boppart; Stephen A. Boppart M.D.

doi:10.1117/12.874183

28 February 2011 Long-term time-lapse multimodal microscopy for tracking cell dynamics in live tissue

Benedikt W. Graf, Maria C. Valero, Eric J. Chaney, Marina Marjanovic, Marni D. Boppart, Stephen A. Boppart M.D.

Proceedings Volume 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX; 790206 (2011) https://doi.org/10.1117/12.874183
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

High speed intravital microscopy has emerged as an essential tool for studying cellular dynamics in live tissue. A limitation of this technique, however, is that the timescale that a sample can be continuously imaged is limited by practical considerations to several hours. Long term observation of live tissue is of great interest for a variety of research areas. We present methods for observing long term cellular dynamics in live tissue based on three-dimensional registration of time-lapse intravital microscopy images. For these experiments we utilized a custom multimodal microscope that allows simultaneous and co-registered acquisition of optical coherence (OCM) and multiphoton (MPM) microscopy images. OCM allows the structure of a sample to be visualized based on backscattered light while MPM excited fluorescence allows individual cells and cell function to be visualized. The OCM images of tissue structure are used to register data sets taken at different time points. The transformations of the OCM images are applied to MPM images to determine the migration of cell populations. This method of image registration is applied to in vivo tracking of bone-marrow derived GFP-labeled stem cells in mouse skin following bone marrow transplants from GFP donors into species-matched wildtype hosts. The use of three-dimensional image registration of time-lapse microscopy images enables tracking these cells after local cutaneous injury, and for investigating the role of skin stem cells in wound healing.

Citation Download Citation

Benedikt W. Graf, Maria C. Valero, Eric J. Chaney, Marina Marjanovic, Marni D. Boppart, and Stephen A. Boppart M.D. "Long-term time-lapse multimodal microscopy for tracking cell dynamics in live tissue", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 790206 (28 February 2011); https://doi.org/10.1117/12.874183

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