Behavior of optical properties of coagulated blood sample at 633-nm wavelength

Beatriz Morales Cruzado; Sergio Vázquez y Montiel; José Alberto Delgado Atencio

doi:10.1117/12.873591

22 February 2011 Behavior of optical properties of coagulated blood sample at 633 nm wavelength

Beatriz Morales Cruzado, Sergio Vázquez y Montiel, José Alberto Delgado Atencio

Proceedings Volume 7897, Optical Interactions with Tissue and Cells XXII; 78970S (2011) https://doi.org/10.1117/12.873591
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Determination of tissue optical parameters is fundamental for application of light in either diagnostics or therapeutical procedures. However, in samples of biological tissue in vitro, the optical properties are modified by cellular death or cellular agglomeration that can not be avoided. This phenomena change the propagation of light within the biological sample. Optical properties of human blood tissue were investigated in vitro at 633 nm using an optical setup that includes a double integrating sphere system. We measure the diffuse transmittance and diffuse reflectance of the blood sample and compare these physical properties with those obtained by Monte Carlo Multi-Layered (MCML). The extraction of the optical parameters: absorption coefficient μ_a, scattering coefficient _μs and anisotropic factor g from the measurements were carried out using a Genetic Algorithm, in which the search procedure is based in the evolution of a population due to selection of the best individual, evaluated by a function that compares the diffuse transmittance and diffuse reflectance of those individuals with the experimental ones. The algorithm converges rapidly to the best individual, extracting the optical parameters of the sample. We compare our results with those obtained by using other retrieve procedures. We found that the scattering coefficient and the anisotropic factor change dramatically due to the formation of clusters.

Citation Download Citation

Beatriz Morales Cruzado, Sergio Vázquez y Montiel, and José Alberto Delgado Atencio "Behavior of optical properties of coagulated blood sample at 633 nm wavelength", Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 78970S (22 February 2011); https://doi.org/10.1117/12.873591

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available