Three-dimensional functional imaging of lung parenchyma using optical coherence tomography combined with confocal fluorescence microscopy

Maria Gaertner; Peter Cimalla; Lilla Knels; Sven Meissner; Edmund Koch

doi:10.1117/12.874795

11 February 2011 Three-dimensional functional imaging of lung parenchyma using optical coherence tomography combined with confocal fluorescence microscopy

Maria Gaertner, Peter Cimalla, Lilla Knels, Sven Meissner, Edmund Koch

Author Affiliations +

Proceedings Volume 7889, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV; 78890C (2011) https://doi.org/10.1117/12.874795
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Optical coherence tomography (OCT), as a non-invasive technique for studying tissue morphology, is widely used in in vivo studies, requiring high resolution and fast three-dimensional imaging. Based on light scattering it reveals micrometer sized substructures of the samples due to changes in their optical properties and therefore allows quantification of the specimen's geometry. Utilizing fluorescence microscopy further information can be obtained from molecular compositions embedded in the investigated object. Fluorescent markers, specifically binding to the substance of interest, reveal the sample's chemical structure and give rise to functional studies. This research presents the application of a combined OCT and laser scanning confocal microscopy (LSCM) system to investigate structural details in lung tissue. OCT reveals the three-dimensional morphology of the alveoli whereas fluorescence detection, arising from the fluorophore Sulforhodamin B (SRB) which is binding to elastin, shows the elastic meshwork of the organs extracellular matrix. Different plains of fluorescence can be obtained by using a piezo driven objective and exploiting the confocal functionality of the setup. Both techniques combined in one optical system not only ease the experimental procedure but also contribute to a thorough description of tissue's morphology and chemical composition.

Citation Download Citation

Maria Gaertner, Peter Cimalla, Lilla Knels, Sven Meissner, and Edmund Koch "Three-dimensional functional imaging of lung parenchyma using optical coherence tomography combined with confocal fluorescence microscopy", Proc. SPIE 7889, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV, 78890C (11 February 2011); https://doi.org/10.1117/12.874795

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