Determination of blood plasma fluorescence extinction coefficients for dyes used in three-compartment binding model

Kimberley S. Samkoe; Kristian Sexton; Kenneth Tichauer; Scott C. Davis; Julia A. O'Hara; Tayyaba Hasan; Brian W. Pogue

doi:10.1117/12.875408

10 February 2011 Determination of blood plasma fluorescence extinction coefficients for dyes used in three-compartment binding model

Kimberley S. Samkoe, Kristian Sexton, Kenneth Tichauer, Scott C. Davis, Julia A. O'Hara, Tayyaba Hasan, Brian W. Pogue

Author Affiliations +

Proceedings Volume 7886, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX; 78860A (2011) https://doi.org/10.1117/12.875408
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

A three-compartment kinetic model for the binding of a ligand to its receptor in tumor tissue has been explained and the kinetic rates of the model are currently being investigated. In order to determine the plasma excretion rates of the dyes of interest, the fluorescence extinction coefficients must be determined. The fluorescence extinction coefficients of the IRDye700DX-carboxylate (IRDye700DX-C) and IRDye800CW-conjugated to EGFR (IRDye800CW-EGF) have been to be 7.98 ×10⁶ μM^-1 cm^-1 and 4.73x10⁶ μM^-1 cm^-1, respectively. We determined that the linear range of these dyes in the blood plasma of a mouse was 0 - 0.26 μM. Administration of 1 nmol of each of these dyes to a mouse weighing 25-30g (0.04 μM - 0.033 μM, respectively) will result in blood plasma fluorescence in the linear and readable range.

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Kimberley S. Samkoe, Kristian Sexton, Kenneth Tichauer, Scott C. Davis, Julia A. O'Hara, Tayyaba Hasan, and Brian W. Pogue "Determination of blood plasma fluorescence extinction coefficients for dyes used in three-compartment binding model", Proc. SPIE 7886, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX, 78860A (10 February 2011); https://doi.org/10.1117/12.875408

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