Fluorescence lifetime imaging of skin cancer

Rakesh Patalay; Clifford Talbot; Ian Munro; Hans Georg Breunig; Karsten König; Yuri Alexandrov; Sean Warren; Mark A. A. Neil; Paul M. W. French; Anthony Chu; Gordon W. Stamp; Chris Dunsby

doi:10.1117/12.873298

17 February 2011 Fluorescence lifetime imaging of skin cancer

Rakesh Patalay, Clifford Talbot, Ian Munro, Hans Georg Breunig, Karsten König, Yuri Alexandrov, Sean Warren, Mark A. A. Neil, Paul M. W. French, Anthony Chu, Gordon W. Stamp, Chris Dunsby

Author Affiliations +

Proceedings Volume 7883, Photonic Therapeutics and Diagnostics VII; 78830A (2011) https://doi.org/10.1117/12.873298
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Fluorescence intensity imaging and fluorescence lifetime imaging microscopy (FLIM) using two photon microscopy (TPM) have been used to study tissue autofluorescence in ex vivo skin cancer samples. A commercially available system (DermaInspect®) was modified to collect fluorescence intensity and lifetimes in two spectral channels using time correlated single photon counting and depth-resolved steady state measurements of the fluorescence emission spectrum. Uniquely, image segmentation has been used to allow fluorescence lifetimes to be calculated for each cell. An analysis of lifetime values obtained from a range of pigmented and non-pigmented lesions will be presented.

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Rakesh Patalay, Clifford Talbot, Ian Munro, Hans Georg Breunig, Karsten König, Yuri Alexandrov, Sean Warren, Mark A. A. Neil, Paul M. W. French, Anthony Chu, Gordon W. Stamp, and Chris Dunsby "Fluorescence lifetime imaging of skin cancer", Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 78830A (17 February 2011); https://doi.org/10.1117/12.873298

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