Determination of singlet oxygen quantum yield of HiPorfin using Singlet Oxygen Sensor Green

Huiyun Lin; Yi Shen; Defu Chen; Lisheng Lin; Buhong Li; Shusen Xie

doi:10.1117/12.870108

8 November 2010 Determination of singlet oxygen quantum yield of HiPorfin using Singlet Oxygen Sensor Green

Huiyun Lin, Yi Shen, Defu Chen, Lisheng Lin, Buhong Li, Shusen Xie

Author Affiliations +

Proceedings Volume 7845, Optics in Health Care and Biomedical Optics IV; 78451J (2010) https://doi.org/10.1117/12.870108
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

Singlet oxygen (¹O₂) is widely considered to be the predominant cytotoxic agent for photodynamic therapy (PDT), and the ¹O₂ quantum yield is a key characteristic for the newly developed photosensitizers. In this study, ¹O₂ generation from the HiPorfin is monitored indirectly by using the fluorescence probe Singlet Oxygen Sensor Green (SOSG). The good linear relationship between the concentration of HiPorfin and the initial reaction rate of SOSG is observed, and the ¹O₂ quantum yield of HiPorfin is determined to be 0.124 ± 0.001, as compared to the standard reference of Rose Bengal. The results suggest that SOSG can be useful for ¹O₂ quantum yield determination for the potential photosensitizers in PDT.

Citation Download Citation

Huiyun Lin, Yi Shen, Defu Chen, Lisheng Lin, Buhong Li, and Shusen Xie "Determination of singlet oxygen quantum yield of HiPorfin using Singlet Oxygen Sensor Green", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78451J (8 November 2010); https://doi.org/10.1117/12.870108

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