In-vitro micro-Raman study of tissue samples for detecting cervical and ovarian cancer with 785-nm laser excitation

S. K. Sharma; L. E. Kamemoto; A. K. Misra; M. T. Goodman; H. W. Luk; J. L. Killeen

doi:10.1117/12.849613

23 April 2010 In-vitro micro-Raman study of tissue samples for detecting cervical and ovarian cancer with 785-nm laser excitation

S. K. Sharma, L. E. Kamemoto, A. K. Misra, M. T. Goodman, H. W. Luk, J. L. Killeen

Author Affiliations +

Proceedings Volume 7674, Smart Biomedical and Physiological Sensor Technologies VII; 76740I (2010) https://doi.org/10.1117/12.849613
Event: SPIE Defense, Security, and Sensing, 2010, Orlando, Florida, United States

Abstract

We present results of in vitro micro-Raman spectroscopy of normal and cancerous cervical and ovarian tissues excited with 785 nm near-infrared (NIR) laser. Micro- Raman spectra of squamous cervical cells of both cervix and ovarian tissues show significant differences in the spectra of normal and cancerous cells. In particular, several well-defined Raman peaks in the 775-975 cm^-1 region are observed in the spectra of normal cervix squamous cells but are completely missing in the spectra of invasive cervical cancer cells. In the high-frequency 2800-3100 cm^-1 region it is shown that the peak area under CH stretching band is much lower than the corresponding area in the spectra of normal cells. In the case of ovarian tissues, the micro-Raman spectra show noticeable spectral differences between normal cells and ovarian serous cancer cells. In particular, we observed the accumulation of β-carotene in ovarian serous cancer cells compared to normal ovarian cells from women with no ovarian cancer. The NIR micro-Raman spectroscopy offers a potential molecular technique for detecting cervical and ovarian cancer from the respective tissues.

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S. K. Sharma, L. E. Kamemoto, A. K. Misra, M. T. Goodman, H. W. Luk, and J. L. Killeen "In-vitro micro-Raman study of tissue samples for detecting cervical and ovarian cancer with 785-nm laser excitation", Proc. SPIE 7674, Smart Biomedical and Physiological Sensor Technologies VII, 76740I (23 April 2010); https://doi.org/10.1117/12.849613

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