Density amplification in laser-assisted protein adsorption by photobleaching

Jonathan M. Bélisle; Santiago Costantino

doi:10.1117/12.841997

17 February 2010 Density amplification in laser-assisted protein adsorption by photobleaching

Jonathan M. Bélisle, Santiago Costantino

Proceedings Volume 7584, Laser Applications in Microelectronic and Optoelectronic Manufacturing XV; 75840O (2010) https://doi.org/10.1117/12.841997
Event: SPIE LASE, 2010, San Francisco, California, United States

Abstract

Spatial distributions of proteins are crucial for development, growth and normal life of organisms. Position of cells in a morphogen gradient determines their differentiation in a specific manner. Neutrophils are the initial responders to bacterial infection or other inflammatory stimuli and have the ability to migrate rapidly up shallow gradients of attractants in vivo. Moreover, for the correct wiring of the nervous system, axonal growth cones detect concentration changes of specific proteins called guidance cues to navigate and reach their targets. Guidance cues can either be chemoattractive or chemorepulsive, and the same protein can act successively as both depending on the time point in development or the simultaneous presence of other molecules. A prerequisite to understand chemotaxis in a precise manner is the availability of a method able to reproduce in vitro the spatial distributions of proteins found in vivo. We recently introduced LAPAP (Laser-assisted protein adsoption by photobleaching), an optical method to produce substrate-bound protein patterns with micron resolution. Here, we present how the amount of protein present on the pattern can be increased by one order of magnitude.

Citation Download Citation

Jonathan M. Bélisle and Santiago Costantino "Density amplification in laser-assisted protein adsorption by photobleaching", Proc. SPIE 7584, Laser Applications in Microelectronic and Optoelectronic Manufacturing XV, 75840O (17 February 2010); https://doi.org/10.1117/12.841997

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