Smart pH cuvette for optical monitoring of pH of biological samples

Derek A. Guenther; Mahmoud R. Shahriari

doi:10.1117/12.840670

16 February 2010 Smart pH cuvette for optical monitoring of pH of biological samples

Proceedings Volume 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II; 75761A (2010) https://doi.org/10.1117/12.840670
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A Smart pH Cuvette is developed by coating the inner surface with pH sensitive thin film. The coating is a hydroscopic sol-gel material doped with colorimetric pH indicator dye sensitive to the pH of analyte solutions in biological range. Ocean optics miniaturized spectrometers are used for signal detection and analysis, along with multimode optical fibers. This new pH sensing arrangement yields an inexpensive solution for monitoring the pH of samples for biological applications. The Smart pH Cuvettes provide a resolution of 0.01 pH units, an accuracy of 1% of the reading, and 90% response in less than 10 seconds.

Citation Download Citation

Derek A. Guenther and Mahmoud R. Shahriari "Smart pH cuvette for optical monitoring of pH of biological samples", Proc. SPIE 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II, 75761A (16 February 2010); https://doi.org/10.1117/12.840670

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