Low-cost fluorescence microscopy for point-of-care cell imaging

Michael J. Lochhead; Jeff Ives; Monique Givens; Marie Delaney; Kevin Moll; Christopher J. Myatt

doi:10.1117/12.842099

25 February 2010 Low-cost fluorescence microscopy for point-of-care cell imaging

Michael J. Lochhead, Jeff Ives, Monique Givens, Marie Delaney, Kevin Moll, Christopher J. Myatt

Proceedings Volume 7572, Optical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics; 75720B (2010) https://doi.org/10.1117/12.842099
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Fluorescence microscopy has long been a standard tool in laboratory medicine. Implementation of fluorescence microscopy for near-patient diagnostics, however, has been limited due to cost and complexity associated with traditional fluorescence microscopy techniques. There is a particular need for robust, low-cost imaging in high disease burden areas in the developing world, where access to central laboratory facilities and trained staff is limited. Here we describe a point-of-care assay that combines a disposable plastic cartridge with an extremely low cost fluorescence imaging instrument. Based on a novel, multi-mode planar waveguide configuration, the system capitalizes on advances in volume-manufactured consumer electronic components to deliver an imaging system with minimal moving parts and low power requirements. A two-color cell imager is presented, with magnification optimized for enumeration of immunostained human T cells. To demonstrate the system, peripheral blood mononuclear cells were stained with fluorescently labeled anti-human-CD4 and anti-human-CD3 antibodies. Registered images were used to generate fractional CD4+ and CD3+ staining and enumeration results that show excellent correlation with flow cytometry. The cell imager is under development as a very low cost CD4+ T cell counter for HIV disease management in limited resource settings.

Citation Download Citation

Michael J. Lochhead, Jeff Ives, Monique Givens, Marie Delaney, Kevin Moll, and Christopher J. Myatt "Low-cost fluorescence microscopy for point-of-care cell imaging", Proc. SPIE 7572, Optical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics, 75720B (25 February 2010); https://doi.org/10.1117/12.842099

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available