Low-cost two-photon microscope with fully customized trajectories

Stefano Lodo; Alessandra Tomaselli; Carla Vacchi; Elena Ugolotti

doi:10.1117/12.841631

25 February 2010 Low-cost two-photon microscope with fully customized trajectories

Stefano Lodo, Alessandra Tomaselli, Carla Vacchi, Elena Ugolotti

Proceedings Volume 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII; 757016 (2010) https://doi.org/10.1117/12.841631
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A modular and efficient nonlinear scanning microscope using an ultrashort pulse laser has been developed. The system is fully supervised by with an ad-hoc electronic system based on FPGA (Field Programmable Gate Array). A closed-loop control allows the compensation of scanning system non-idealities. Fully customizable trajectories can be used, in order to find best performances of the mechanical system. The electronic system is also characterized by the management of the target focusing on different focal planes and automatic research of the best focal plane. An ad-hoc software controls the system by a standard USB interface and processes images.

Citation Download Citation

Stefano Lodo, Alessandra Tomaselli, Carla Vacchi, and Elena Ugolotti "Low-cost two-photon microscope with fully customized trajectories", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 757016 (25 February 2010); https://doi.org/10.1117/12.841631

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