Fully automated data acquisition and fast data interpretation in a customized multimodal multiphoton microscope

C. D. Rahn; H. Meine; S. Gallinat; H. Wenck; K. P. Wittern; F. Fischer

doi:10.1117/12.840893

25 February 2010 Fully automated data acquisition and fast data interpretation in a customized multimodal multiphoton microscope

C. D. Rahn, H. Meine, S. Gallinat, H. Wenck, K. P. Wittern, F. Fischer

Author Affiliations +

Proceedings Volume 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII; 757015 (2010) https://doi.org/10.1117/12.840893
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Examining a volunteer or patient in-vivo usually is a strictly time-constrained process. When complex (custom) multicomponent, multi-modal imaging devices are involved, an examination session might be significantly stressful for the experimenter who is in charge of instrumental control. This is especially true when many parameters, several different devices or several different software applications are to be controlled during a single session. Stress may increase the probability of suboptimal data acquisition and data loss. The experimenter should be focused on the sample, not on instrumental control. In order to ease image acquisition, we present concepts for a new intuitive and interactive software application that integrates control of all hardware components, guides the experimenter through customizable acquisition workflows, presents the recorded data ready for facilitated interpretation using state-of-the-art image processing algorithms and thus supports the experimenter in acquiring optimized and highly valuable image data. Our concept combines into a single application many functions that were previously performed by several different programs, it allows linking together the functionalities, it allows automation, and it adds several convenience and safety features.

Citation Download Citation

C. D. Rahn, H. Meine, S. Gallinat, H. Wenck, K. P. Wittern, and F. Fischer "Fully automated data acquisition and fast data interpretation in a customized multimodal multiphoton microscope", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 757015 (25 February 2010); https://doi.org/10.1117/12.840893

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