Polarization-resolved coherent anti-Stokes Raman scattering microscopy

Fabiana Munhoz; Sophie Brustlein; Sophie Brasselet; Hervé Rigneault

doi:10.1117/12.840852

26 February 2010 Polarization-resolved coherent anti-Stokes Raman scattering microscopy

Fabiana Munhoz, Sophie Brustlein, Sophie Brasselet, Hervé Rigneault

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75690P (2010) https://doi.org/10.1117/12.840852
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We have implemented a polarization-resolved coherent anti-Stokes Raman scattering (CARS) microscopy, based on the continuous variation of the incident linear polarization at the pump and Stokes wavelengths, together with a polarized analysis of the anti-Stokes signal. In isotropic media, such as solutions, this technique can be a powerful way to probe microscopic-scale information, such as the vibrational symmetry properties of the molecular bonds. In ordered media, additional macroscopic-scale structural information can be obtained, such as the orientation of the unit-cell of a crystal in 3D.

Citation Download Citation

Fabiana Munhoz, Sophie Brustlein, Sophie Brasselet, and Hervé Rigneault "Polarization-resolved coherent anti-Stokes Raman scattering microscopy", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75690P (26 February 2010); https://doi.org/10.1117/12.840852

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