Development of in vivo confocal microscope for reflection and fluorescence imaging simultaneously

MyoungKi Ahn; ByungSeon Chun; Cheol Song; DaeGab Gweon

doi:10.1117/12.841223

25 February 2010 Development of in vivo confocal microscope for reflection and fluorescence imaging simultaneously

MyoungKi Ahn, ByungSeon Chun, Cheol Song, DaeGab Gweon

Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 756821 (2010) https://doi.org/10.1117/12.841223
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

In-vivo confocal microscope technology can be applied to the medical imaging diagnosis and new drug development. We present an in-vivo confocal microscope that can acquire a reflection image and a fluorescence image simultaneously and independently. To obtain reflection confocal images, we used a linearly polarized diode laser with the wavelength of 830 nm. To acquire fluorescence confocal images, we used two diode lasers with the wavelength of 488 nm and 660 nm, respectively. Because of a broad wavelength bandwidth from visible (488 nm) to near-IR (830 nm), we designed and optimized the optical system to reduce various optical aberrations. With the developed in-vivo confocal microscope, we performed ex-vivo cell imaging and in-vivo imaging of the human skin.

Citation Download Citation

MyoungKi Ahn, ByungSeon Chun, Cheol Song, and DaeGab Gweon "Development of in vivo confocal microscope for reflection and fluorescence imaging simultaneously", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756821 (25 February 2010); https://doi.org/10.1117/12.841223

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