DNA biosensors based on layer-by-layer self-assembled multilayer films of carbon nanotubes and gold nanoparticles

Yiyun Xiao; Zhao Dai; Jimei Zhang; Jiechun Pang; Shichao Xu; Guo Zheng

doi:10.1117/12.840316

20 October 2009 DNA biosensors based on layer-by-layer self-assembled multilayer films of carbon nanotubes and gold nanoparticles

Yiyun Xiao, Zhao Dai, Jimei Zhang, Jiechun Pang, Shichao Xu, Guo Zheng

Author Affiliations +

Proceedings Volume 7493, Second International Conference on Smart Materials and Nanotechnology in Engineering; 74935A (2009) https://doi.org/10.1117/12.840316
Event: Second International Conference on Smart Materials and Nanotechnology in Engineering, 2009, Weihai, China

Abstract

A novel DNA biosensor based on layer-by-layer self-assembled multi-walled carbon nanotubes (MWNTs) and gold nano-particles (GNPs) was presented in this paper, in which the probe HS-ssDNA oligonucleotides, MWNTs and GNPs were all covalently immobilized by chemical Au-Sulphide bonding. Firstly, the super short MWNTs were prepared and modified with thio groups which could be self-assembled onto the surface of Au elcetrode by Au-sulphide bonding, then the GNPs were chemically adhered to the surfaces of MWNTs by forming Au-sulphide bonding again, at last the selfassamble of probe DNA oligonucleotides were also covalently immobilized via Au-sulphide bonding between thio groups at the ends of the DNA oligonucleotides and GNPs. Hybridization between the probe HS-ssDNA oligonucleotides and target DNA oligonucleotides was confirmed by the changes in the voltammetric peak of an anionic intercalator, anthraquinone-2,6-disulfonic acid (AQDS) as a hybridization indicator. The cyclic voltammetric and differential pulse voltammetry responses demonstrated that the DNA biosensors based on Layer-by-layer self-assembled multilayer films of MWNTs and NGPs offer a higher hybridization efficiency and selectivity compared to those based on only random MWNTs or GNPs.

Citation Download Citation

Yiyun Xiao, Zhao Dai, Jimei Zhang, Jiechun Pang, Shichao Xu, and Guo Zheng "DNA biosensors based on layer-by-layer self-assembled multilayer films of carbon nanotubes and gold nanoparticles", Proc. SPIE 7493, Second International Conference on Smart Materials and Nanotechnology in Engineering, 74935A (20 October 2009); https://doi.org/10.1117/12.840316

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