Antigen detection at atomolar concentration using optical tweezers

Mathieu Laliberté; François Bordeleau; Normand Marceau; Yunlong Sheng

doi:10.1117/12.839769

4 August 2009 Antigen detection at atomolar concentration using optical tweezers

Mathieu Laliberté, François Bordeleau, Normand Marceau, Yunlong Sheng

Proceedings Volume 7386, Photonics North 2009; 738609 (2009) https://doi.org/10.1117/12.839769
Event: Photonics North 2009, 2009, Quebec, Canada

Abstract

Methods that avoid intermediate amplification steps to detect protein markers of pathological disturbances would be of wide interest in the clinical environment. This is particularly the case in cancer diagnosis, where protein fragments are released into the blood by the emerging cancer cells. These fragments generate an antigen-antibody reaction, and the concentration of the antigen is known to modulate this interaction. Here we report on the development of a novel optical tweezers-based procedure to measure minute amount of antigen in a biological fluid. The force was applied on a 3μm polystyrene bead coated with Bovine Serum Albumin (BSA) attached on a 1.5 μm diameter borosilicate rod tip coated with anti-BSA antibody. First, we verified that the binding strength was dependent on the protein concentration on the bead. We then assessed the sensitivity range by finding the minimal BSA concentration in solution that can still interfere with the bead-rod linkage. On the whole, the results demonstrated that proteinous antigen present in a biological fluid could possibly be detectable at atomolar concentration through the use of an optical tweezers.

Citation Download Citation

Mathieu Laliberté, François Bordeleau, Normand Marceau, and Yunlong Sheng "Antigen detection at atomolar concentration using optical tweezers", Proc. SPIE 7386, Photonics North 2009, 738609 (4 August 2009); https://doi.org/10.1117/12.839769

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