Detection of CD4+ and CD8 + T-lymphocytes with the optofluidic ring resonator (OFRR) biosensor

John T. Gohring; Xudong Fan

doi:10.1117/12.818255

29 April 2009 Detection of CD4+ and CD8 + T-lymphocytes with the optofluidic ring resonator (OFRR) biosensor

John T. Gohring, Xudong Fan

Proceedings Volume 7322, Photonic Microdevices/Microstructures for Sensing; 73220D (2009) https://doi.org/10.1117/12.818255
Event: SPIE Defense, Security, and Sensing, 2009, Orlando, Florida, United States

Abstract

We have demonstrated the use of the Opto-Fluidic ring resonator (OFRR) to achieve the label-free detection of CD4+ and CD8+ T-Lymphocytes. The OFRR sensing technology combines microfluidics and optical sensing in a small platform that achieves rapid detection. In this work, white blood cells were obtained from healthy blood and the concentration altered to reflect CD4 and CD8 concentrations of HIV infected individuals. The OFRR was modified to effectively capture these receptors located on T-Lymphocytes and obtain a sensing signal through interaction with an evanescent field. Results show isolation of CD4+ and CD8+ T-Lymphocytes at medically significant levels. This work will lead to a device that can provide a CD4 and CD8 count to measure HIV progression in a low cost sensing setup.

Citation Download Citation

John T. Gohring and Xudong Fan "Detection of CD4+ and CD8 + T-lymphocytes with the optofluidic ring resonator (OFRR) biosensor", Proc. SPIE 7322, Photonic Microdevices/Microstructures for Sensing, 73220D (29 April 2009); https://doi.org/10.1117/12.818255

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