The effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca2+]i in the cells

YuBin Ji; ShiYong Gao; ChenFeng Ji; Xiang Zou

doi:10.1117/12.821308

6 March 2009 The effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca²⁺]i in the cells

YuBin Ji, ShiYong Gao, ChenFeng Ji, Xiang Zou

Proceedings Volume 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine; 72800Q (2009) https://doi.org/10.1117/12.821308
Event: Photonics and Optoelectronics Meetings, 2008, Wuhan, China

Abstract

To observe the effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca²⁺]i in the cells, and to uncover the mechanism by which solanine induces apoptosis. HepG2 cells are double stained with and Fluo-3/AM, and both the change in membrane potential of mitochondria and that of [Ca²⁺]i in the cells are observed using LCSM. The results of double staining with TMRE and Fluo-3/AM show that solanine can lower membrane potential and increase the concentration of Ca²⁺ in the cells Solanine opens up the PT channels in the membrane by lowering the membrane potential, leading to Ca²⁺ being transported down its concentration gradient, which in turn leads to the rise of the concentration of Ca²⁺ in the cell, turning on the mechanism for apoptosis.

Citation Download Citation

YuBin Ji, ShiYong Gao, ChenFeng Ji, and Xiang Zou "The effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca²⁺]i in the cells", Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72800Q (6 March 2009); https://doi.org/10.1117/12.821308

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