Microfluidic chip based hematoanalyzer using polyelectrolytic gel electrodes

Kwang Bok Kim; Honggu Chun; Hee Chan Kim; Taek Dong Chung

doi:10.1117/12.810648

23 February 2009 Microfluidic chip based hematoanalyzer using polyelectrolytic gel electrodes

Kwang Bok Kim, Honggu Chun, Hee Chan Kim, Taek Dong Chung

Proceedings Volume 7207, Microfluidics, BioMEMS, and Medical Microsystems VII; 72070S (2009) https://doi.org/10.1117/12.810648
Event: SPIE MOEMS-MEMS: Micro- and Nanofabrication, 2009, San Jose, California, United States

Abstract

We reports on a novel microfluidic chip with polyelectrolytic gel electrodes (PGEs) used to rapidly count the number of red blood cells in diluted whole blood. The number and amplitude of dc impedance peaks provide the information about the number and size of red blood cells, respectively. This system features a low-voltage dc detection method and noncontact condition between cells and metal electrodes. The performance of this PGEs-based system was evaluated in three steps. First, in order to observe the size-only dependence of the impedance signal, three different sizes of fluorescent microbeads were used in the experiment. Second, the cell counting performance was evaluated by using 7.2 μm fluorescent microbeads, similar in size to red blood cells, in various concentrations and comparing the results with an animal hematoanalyzer. Finally, in human blood sample tests, intravenously collected whole blood was just diluted in a phosphate buffered saline without centrifuge or other pretreatments. The PGEs-based system produced almost identical numbers of red blood cells in over 800-fold diluted samples to the results from a commercialized human hematoanalyzer.

Citation Download Citation

Kwang Bok Kim, Honggu Chun, Hee Chan Kim, and Taek Dong Chung "Microfluidic chip based hematoanalyzer using polyelectrolytic gel electrodes", Proc. SPIE 7207, Microfluidics, BioMEMS, and Medical Microsystems VII, 72070S (23 February 2009); https://doi.org/10.1117/12.810648

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KEYWORDS

Blood

Electrodes

Glasses

Microfluidics

Metals

Ultraviolet radiation

Head-mounted displays

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