Patterning of fibronectin using laser writer for force measurement in cells

B. Codan; T. Gaiotto; R. Di Niro; R. Marzari; V. Sergo

doi:10.1117/12.809698

16 February 2009 Patterning of fibronectin using laser writer for force measurement in cells

B. Codan, T. Gaiotto, R. Di Niro, R. Marzari, V. Sergo

Proceedings Volume 7188, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VI; 71880P (2009) https://doi.org/10.1117/12.809698
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Mechanical forces and living cells are closely related, in particular in connections, named Focal Adhesions (FAs), between cells and extracellular matrix. FAs are mechanosensors and regulate physiological processes. The aim of this contribution is to explore the possibility of performing micrometric and submicrometric protein pattern in order to study FAs on different materials. Typical substrates for microelectromechanical systems (MEMS) are tested and the results are reported. A laser is used to produce a pattern of extracellular matrix proteins, like fibronectin. A new use of a Raman microprobe is described. Cells arrange in a regular shape, following the geometry of the pattern. Protein spots last for more than 24 hours. It is very important to have a complete control on FAs and the technique proposed is a suitable solution.

Citation Download Citation

B. Codan, T. Gaiotto, R. Di Niro, R. Marzari, and V. Sergo "Patterning of fibronectin using laser writer for force measurement in cells", Proc. SPIE 7188, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VI, 71880P (16 February 2009); https://doi.org/10.1117/12.809698

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