Revisiting optical clearing with dimethyl sulfoxide (DMSO): in vitro and in vivo studies

R. Anthony McClure; Charles Stoianovici; Sanjeev Karma; Bernard Choi

doi:10.1117/12.810888

24 February 2009 Revisiting optical clearing with dimethyl sulfoxide (DMSO): in vitro and in vivo studies

R. Anthony McClure, Charles Stoianovici, Sanjeev Karma, Bernard Choi

Proceedings Volume 7187, Biomedical Applications of Light Scattering III; 718707 (2009) https://doi.org/10.1117/12.810888
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Functional optical characterization of disease progression and response to therapy suffers from loss of spatial resolution and imaging depth due to scattering, impacting the ability of researchers to localize and quantify molecular processes. Here we report on the ability of dimethyl sulfoxide (DMSO) to reduce temporarily the optical scattering of skin. Data collected from in vitro phantom images and in vivo fluorescence images demonstrate the potential of this simple method to mitigate the blurring effects of scattering with topical application, which we expect will improve the accuracy and localization of in vivo molecular imaging studies.

Citation Download Citation

R. Anthony McClure, Charles Stoianovici, Sanjeev Karma, and Bernard Choi "Revisiting optical clearing with dimethyl sulfoxide (DMSO): in vitro and in vivo studies", Proc. SPIE 7187, Biomedical Applications of Light Scattering III, 718707 (24 February 2009); https://doi.org/10.1117/12.810888

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