Action potential detection by non-linear microscopy

Leonardo Sacconi; Jacopo Lotti; Rodney P. O’Connor; Jonathan Mapelli; Daniela Gandolfi; Egidio D'Angelo; Francesco S. Pavone

doi:10.1117/12.807966

23 February 2009 Action potential detection by non-linear microscopy

Leonardo Sacconi, Jacopo Lotti, Rodney P. O’Connor, Jonathan Mapelli, Daniela Gandolfi, Egidio D'Angelo, Francesco S. Pavone

Author Affiliations +

Proceedings Volume 7161, Photonic Therapeutics and Diagnostics V; 71613F (2009) https://doi.org/10.1117/12.807966
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

In this work, we combined the advantages of second-harmonic generation (SHG) with a random access (RA) excitation scheme to realize a new microscope (RA-SHG) capable of optically recording fast membrane potential events occurring in a wide-field configuration. The RA-SHG microscope in combination with a bulk staining method with FM4-64 was used to simultaneously record electrical activity from clusters of Purkinje cells (PCs) in acute cerebellar slices. Spontaneous electrical activity was also monitored simultaneously in pairs of neurons, where APs were recorded in a single trial without averaging. These results show the strength of this technique to describe the temporal dynamics of neuronal assemblies.

Citation Download Citation

Leonardo Sacconi, Jacopo Lotti, Rodney P. O’Connor, Jonathan Mapelli, Daniela Gandolfi, Egidio D'Angelo, and Francesco S. Pavone "Action potential detection by non-linear microscopy", Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 71613F (23 February 2009); https://doi.org/10.1117/12.807966

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