Fourier transform infrared spectroscopy of deuterated proteins

A. Marcano O.; Y. Markushin; N. Melikechi; D. Connolly

doi:10.1117/12.794878

2 September 2008 Fourier transform infrared spectroscopy of deuterated proteins

A. Marcano O., Y. Markushin, N. Melikechi, D. Connolly

Proceedings Volume 7049, Linear and Nonlinear Optics of Organic Materials VIII; 70490Z (2008) https://doi.org/10.1117/12.794878
Event: Photonic Devices + Applications, 2008, San Diego, California, United States

Abstract

We report on Fourier transform spectra of deuterated proteins: Bovine Serum Albumin, Leptin, Insulin-like Growth Factor II, monoclonal antibody to ovarian cancer antigen CA125 and Osteopontin. The spectra exhibit changes in the relative amplitude and spectral width of certain peaks. New peaks not present in the non-deuterated sample are also observed. Ways for improving the deuteration of proteins by varying the temperature and dilution time are discussed. We propose the use of deuterated proteins to increase the sensitivity of immunoassays aimed for early diagnostic of diseases most notably cancer.

Citation Download Citation

A. Marcano O., Y. Markushin, N. Melikechi, and D. Connolly "Fourier transform infrared spectroscopy of deuterated proteins", Proc. SPIE 7049, Linear and Nonlinear Optics of Organic Materials VIII, 70490Z (2 September 2008); https://doi.org/10.1117/12.794878

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available