Parallel microfluidic arrays for SPRi detection

Eric Ouellet; Christopher Lausted; Leroy Hood M.D.; Eric T. Lagally

doi:10.1117/12.792765

29 August 2008 Parallel microfluidic arrays for SPRi detection

Eric Ouellet, Christopher Lausted, Leroy Hood M.D., Eric T. Lagally

Proceedings Volume 7035, Biosensing; 703505 (2008) https://doi.org/10.1117/12.792765
Event: NanoScience + Engineering, 2008, San Diego, California, United States

Abstract

Surface Plasmon Resonance imaging (SPRi) is a label-free technique for the quantitation of binding affinities and concentrations for a wide variety of target molecules. Although SPRi is capable of determining binding constants for multiple ligands in parallel, current commercial instruments are limited to a single analyte stream and a limited number of ligand spots. Measurement of target concentration also requires the serial introduction of different target concentrations; such repeated experiments are conducted manually and are therefore time-intensive. Likewise, the equilibrium determination of concentration for known binding affinity requires long times due to diffusion-limited kinetics to a surface-immobilized ligand. We have developed an integrated microfluidic array using soft lithography techniques for SPRi-based detection and determination of binding affinities for DNA aptamers against human alphathrombin. The device consists of 264 element-addressable chambers isolated by microvalves. The resulting 700 pL volumes surrounding each ligand spot promise to decrease measurement time through reaction rate-limited kinetics. The device also contains a dilution network for simultaneous interrogation of up to six different target concentrations, further speeding detection times. Finally, the element-addressable design of the array allows interrogation of multiple ligands against multiple targets.

Citation Download Citation

Eric Ouellet, Christopher Lausted, Leroy Hood M.D., and Eric T. Lagally "Parallel microfluidic arrays for SPRi detection", Proc. SPIE 7035, Biosensing, 703505 (29 August 2008); https://doi.org/10.1117/12.792765

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