Digital holographic microscope with low spatial and temporal coherence of illumination

Hana Janečková; Pavel Kolman; Pavel Veselý; Radim Chmelík

doi:10.1117/12.800392

25 April 2008 Digital holographic microscope with low spatial and temporal coherence of illumination

Hana Janečková, Pavel Kolman, Pavel Veselý, Radim Chmelík

Proceedings Volume 7000, Optical and Digital Image Processing; 70002E (2008) https://doi.org/10.1117/12.800392
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

In this paper we present a newly developed digital transmission holographic microscope. The microscope enables using arbitrarily low coherent illumination (both spatially and temporally) in conjunction with the off-axis holography. The setup of the microscope, its function and the object wave reconstruction procedure are described. The optical sectioning effect, similar to a confocal microscope, resulting from the use of low spatially coherent light source is demonstrated. The microscope has been tailored for studies of living cell dynamics. Time-lapse phase reconstruction series of live cells activities were carried out. The different behavior related to changes in the cell cycle is demonstrated.

Citation Download Citation

Hana Janečková, Pavel Kolman, Pavel Veselý, and Radim Chmelík "Digital holographic microscope with low spatial and temporal coherence of illumination", Proc. SPIE 7000, Optical and Digital Image Processing, 70002E (25 April 2008); https://doi.org/10.1117/12.800392

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