Contrast mechanisms and signal epidetection in THG microscopy of scattering tissues

Nicolas Olivier; Delphine Débarre; Emmanuel Beaurepaire

doi:10.1117/12.783376

9 May 2008 Contrast mechanisms and signal epidetection in THG microscopy of scattering tissues

Nicolas Olivier, Delphine Débarre, Emmanuel Beaurepaire

Proceedings Volume 6991, Biophotonics: Photonic Solutions for Better Health Care; 699114 (2008) https://doi.org/10.1117/12.783376
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

We use a vector field model to analyze the third-harmonic generation (THG) emission patterns for isolated objects illuminated by a Gaussian beam. Simulations and experiments indicate that THG from biological (dielectric) structures is essentially forward-directed, as opposed to e.g. THG from gold particles. We then address the issue of epidetecting forward-emitted light backscattered in a turbid medium. We use Monte Carlo simulations and measurements to analyze the effect of tissue properties (absorption, scattering), and of the geometry of the collecting optics. This analysis provides guidelines for optimizing epidetection in coherent nonlinear microscopy. An erratum is attached.

Citation Download Citation

Nicolas Olivier, Delphine Débarre, and Emmanuel Beaurepaire "Contrast mechanisms and signal epidetection in THG microscopy of scattering tissues", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 699114 (9 May 2008); https://doi.org/10.1117/12.783376

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available