Detection of multidrug-resistant tuberculosis gene using plasmonics nanoprobes

Hsin-Neng Wang; Fei Yan; Yan Zhang; Tuan Vo-Dinh

doi:10.1117/12.761897

21 February 2008 Detection of multidrug-resistant tuberculosis gene using plasmonics nanoprobes

Hsin-Neng Wang, Fei Yan, Yan Zhang, Tuan Vo-Dinh

Proceedings Volume 6869, Plasmonics in Biology and Medicine V; 686903 (2008) https://doi.org/10.1117/12.761897
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

This paper describes the use of plasmonics-based nanoprobes for detection of multidrug-resistant tuberculosis gene. The plasmonics nanoprobe is composed of a silver nanoparticle pre-coated with a stem-loop DNA probe that is tagged with a Raman label at one end of the stem region, while the other end of the probe is covalently conjugated to the nanoparticle via a thiol-silver bond. The loop region is designed to detect a specific target gene sequence. In the absence of target, the Raman label is in close proximity to the metal surface, resulting in an intense SERS signal upon laser excitation. In the presence of the target DNA sequence, hybridization between the target and probe disrupts the stem-loop configuration, separating the Raman label from the metal surface and quenching the SERS signal. In this study, we successfully demonstrated for the first time the feasibility of using plasmonics nanoprobes for the detection of multidrug-resistant tuberculosis gene.

Citation Download Citation

Hsin-Neng Wang, Fei Yan, Yan Zhang, and Tuan Vo-Dinh "Detection of multidrug-resistant tuberculosis gene using plasmonics nanoprobes", Proc. SPIE 6869, Plasmonics in Biology and Medicine V, 686903 (21 February 2008); https://doi.org/10.1117/12.761897

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available