Integrated Raman and angular scattering microscopy (IRAM)

Zachary J. Smith; Andrew J. Berger

doi:10.1117/12.761796

10 March 2008 Integrated Raman and angular scattering microscopy (IRAM)

Zachary J. Smith, Andrew J. Berger

Proceedings Volume 6853, Biomedical Optical Spectroscopy; 68530T (2008) https://doi.org/10.1117/12.761796
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

A microscope system has been constructed that allows simultaneous acquisition of Raman scattering spectra and elastic scattering Fourier-plane data. The Raman scattering channel reports on chemical composition of the microscopic sample while the elastic scattering channel reports on morphological information about the sample. The system has been validated by acquiring data from single polystyrene beads and analyzing the elastic scattering signal using Generalized Lorenz-Mie Theory while comparing the Raman scattering signature to other polystyrene spectra from the literature. Monocytes and neutrophils, two immune cell types, have also been studied and show clear chemical and morphological differences between cell types.

Citation Download Citation

Zachary J. Smith and Andrew J. Berger "Integrated Raman and angular scattering microscopy (IRAM)", Proc. SPIE 6853, Biomedical Optical Spectroscopy, 68530T (10 March 2008); https://doi.org/10.1117/12.761796

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