Investigation of murine vasodynamics by Fourier domain optical coherence tomography

Sven Meißner; Gregor Müller; Julia Walther; Alexander Krüger; Maximiliano Cuevas; Birgit Eichhorn; Ursula Ravens; Henning Morawietz; Edmund Koch

doi:10.1117/12.728163

11 July 2007 Investigation of murine vasodynamics by Fourier domain optical coherence tomography

Sven Meißner, Gregor Müller, Julia Walther, Alexander Krüger, Maximiliano Cuevas, Birgit Eichhorn, Ursula Ravens, Henning Morawietz, Edmund Koch

Author Affiliations +

Proceedings Volume 6627, Optical Coherence Tomography and Coherence Techniques III; 66270D (2007) https://doi.org/10.1117/12.728163
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

In vivo imaging of blood vessels obtain useful insights in characterizing the dynamics of vasoconstriction and vasodilation. Fourier domain optical Coherence Tomography (FD-OCT) imaging technique permits in vivo investigation of blood vessels in their anatomical context without preparation traumata by temporal resolved image stacks. OCT is an optical, contact less imaging technique based on Michelson interferometry of short coherent near infrared light. Particularly by the possibility of a contact-less measurement and the high axial resolution up to 10 microns OCT is superior to an investigation by ultra sound measurement. Furthermore we obtain a high time resolution of vessel dynamic measurements with the used Fourier domain OCT-system by a high A-scan rate [1,22kHz]. In this study the model of saphenous artery was chosen for analyzing function and dynamics. The arteria saphena in the mouse is a suitable blood vessel due to the small inner diameter, a sensitive response to vasoactive stimuli and an advantageous anatomically position. Male wild type mice (C57BL/6) at the age of 8 weeks were fed control or high-fat diet for 10 weeks before analyzing the vasodynamics. The blood vessel was stimulated by dermal application of potassium to induce vasoconstriction or Sodium-Nitroprusside (SNP) to induce vasodilation. The morphology of the a. saphena and vein was determined by 3D image stacks. Time series (72 seconds, 300x512 pixel per frame) of cross-sectional images were analysed using semi automatic image processing software. Time course of dynamic parameters of the vessel was measured.

Citation Download Citation

Sven Meißner, Gregor Müller, Julia Walther, Alexander Krüger, Maximiliano Cuevas, Birgit Eichhorn, Ursula Ravens, Henning Morawietz, and Edmund Koch "Investigation of murine vasodynamics by Fourier domain optical coherence tomography", Proc. SPIE 6627, Optical Coherence Tomography and Coherence Techniques III, 66270D (11 July 2007); https://doi.org/10.1117/12.728163

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available