Improved image-forming optics for transmission optical projection tomography

Yi Wang; Ruikang Wang

doi:10.1117/12.702250

14 February 2007 Improved image-forming optics for transmission optical projection tomography

Yi Wang, Ruikang Wang

Proceedings Volume 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV; 644304 (2007) https://doi.org/10.1117/12.702250
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We demonstrate an improved image-forming optics for transmission optical projection tomography (TOPT), with which the parallel integral throughout an object can be obtained. This is performed by introducing a shutter with an appropriate diameter at the back focus of the objective lens. We evaluate and compare the performances of the improved and the conventional TOPT systems with different parameters to obtain the optimal configuration. The optimal reconstruction is achieved by the improved configuration with α =0.8° ~1.6°, and the spatial resolution reaches 25 μm. The Optimal configuration is validated by TOPT of a phantom sample and a five days chick embryo.

Citation Download Citation

Yi Wang and Ruikang Wang "Improved image-forming optics for transmission optical projection tomography", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 644304 (14 February 2007); https://doi.org/10.1117/12.702250

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available