The influence of NIR femtosecond laser radiation on the viability of 3D stem cell clusters and tumor spheroids

Aisada Uchugonova; Iris Riemann; Frank Stracke; Erwin Gorjup; Ronan LeHarzic; Karsten König

doi:10.1117/12.702586

10 February 2007 The influence of NIR femtosecond laser radiation on the viability of 3D stem cell clusters and tumor spheroids

Aisada Uchugonova, Iris Riemann, Frank Stracke, Erwin Gorjup, Ronan LeHarzic, Karsten König

Author Affiliations +

Proceedings Volume 6442, Multiphoton Microscopy in the Biomedical Sciences VII; 64421Z (2007) https://doi.org/10.1117/12.702586
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Adult human and rat pancreas stem cells as well as tumor spheroids were irradiated with femtosecond laser pulses in the near infrared (NIR) spectral range at high transient GW/cm² and TW/cm² intensities. The cellular response to the laser exposure was probed by the detection of modifications of NAD(P)H autofluorescence, the formation of reactive oxygen species (ROS) and DNA strand breaks (TUNEL-assay), and viability (live/dead test). The results confirm that long-term scanning of stem cells can be performed at appropriate laser exposure parameters without a measurable impact on the cellular metabolism and vitality. In addition, it was proven that a targeted inactivation of a particular single stem cells or a single tumour cell inside a 3D cell cluster using single point illumination at TW/cm² laser intensities can be performed without affecting neighbouring cells. Therefore multiphoton microscopes can be considered as biosafe tools for long-term analysis of stem cells as well as highly precise optical knocking out of single cells within cell clusters and tissues.

Citation Download Citation

Aisada Uchugonova, Iris Riemann, Frank Stracke, Erwin Gorjup, Ronan LeHarzic, and Karsten König "The influence of NIR femtosecond laser radiation on the viability of 3D stem cell clusters and tumor spheroids", Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421Z (10 February 2007); https://doi.org/10.1117/12.702586

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