Intravital multiphoton microscopy for imaging hepatobiliary function

Wen Lo; Yuan Liu; Hsiao-Ching Chen; Shu-Mei Yang; Tzu-Lin Sun; Ling-Ling Chiou; Guan-Tarn Huang; Chen-Yuan Dong; Hsuan-Shu Lee

doi:10.1117/12.699896

10 February 2007 Intravital multiphoton microscopy for imaging hepatobiliary function

Wen Lo, Yuan Liu, Hsiao-Ching Chen, Shu-Mei Yang, Tzu-Lin Sun, Ling-Ling Chiou, Guan-Tarn Huang, Chen-Yuan Dong, Hsuan-Shu Lee

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Proceedings Volume 6442, Multiphoton Microscopy in the Biomedical Sciences VII; 64421R (2007) https://doi.org/10.1117/12.699896
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

In this work, intravital multiphoton microscopy is applied to the imaging of liver metabolism with the least invasiveness. We observed intravital dynamics of the uptake, processing and excretion of the organic anion species, 6-carboxyfluorescein diacetate (6-CFDA) in the hepatobiliary system. This is achieved by the use of multiphoton microscopy and an in vivo hepatic imaging chamber which allows us to image the dynamics of hepatic metabolism. Multiphoton images revealed that the hepatic processing of 6-CFDA is completed within approximately 50 minutes. The images reveal the liver metabolism of the uptake and processing of 6-CFDA from the hepatocytes, and the subsequent excretion into bile canaliculi. Our results suggest that this approach is a promising technique for investigating intravital hepatic physiology, diseases, and metabolism.

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Wen Lo, Yuan Liu, Hsiao-Ching Chen, Shu-Mei Yang, Tzu-Lin Sun, Ling-Ling Chiou, Guan-Tarn Huang, Chen-Yuan Dong, and Hsuan-Shu Lee "Intravital multiphoton microscopy for imaging hepatobiliary function", Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421R (10 February 2007); https://doi.org/10.1117/12.699896

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KEYWORDS

Liver

Multiphoton microscopy

Luminescence

Mode conditioning cables

Microscopes

In vivo imaging

Second-harmonic generation

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