Experimental study of various effects on backward SHG in biotissue

Nenrong Liu; Yishen Qiu; Xianzeng Zhang

doi:10.1117/12.708713

19 February 2007 Experimental study of various effects on backward SHG in biotissue

Nenrong Liu, Yishen Qiu, Xianzeng Zhang

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 64411U (2007) https://doi.org/10.1117/12.708713
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Second harmonic generation (SHG) is a well-known physical phenomenon of important in nonlinear optics, which has been widely used in tissue morphology and pathology. In this thesis, the influences on backward SHG intensity were studied. A two-photon laser scanning confocal microscope coupled with a mode-locked femtosecond Ti: sapphire laser was used to carry out the experiments. Rat-tails were used as experimental objects, there were 40 slices including transverse and longitudinal. All slices were un-dyed. The changes of backward SHG intensities of rat-tails versus the excited wavelengths, excited powers, exploring-depths and so on were obtained. It was clear that different irradiation conditions had evident influences on backward SHG intensity. Subsequently, the backward SHG imaging and two-photon excited fluorescence (TPEF) imaging through two independent channels were also compared preliminarily. This study demonstrates that SHG is a promising tool for more accurate information in biomedicine fields.

Citation Download Citation

Nenrong Liu, Yishen Qiu, and Xianzeng Zhang "Experimental study of various effects on backward SHG in biotissue", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64411U (19 February 2007); https://doi.org/10.1117/12.708713

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