Richardson-Lucy deconvolution for two-photon fluorescence images via non-linear diffusion equation pre-filtering

Hongmin Zhang; Zhitao Zhang; Qingming Luo; Shaoqun Zeng

doi:10.1117/12.701750

7 February 2007 Richardson-Lucy deconvolution for two-photon fluorescence images via non-linear diffusion equation pre-filtering

Hongmin Zhang, Zhitao Zhang, Qingming Luo, Shaoqun Zeng

Author Affiliations +

Proceedings Volume 6436, Complex Dynamics and Fluctuations in Biomedical Photonics IV; 64360P (2007) https://doi.org/10.1117/12.701750
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Two-photon fluorescence microscopy is a powerful technique to obtain the stacks of neuronal individual or population morphologies deep inside brain tissue in vivo. However, the stacks often suffer from increasing noises with depth because of light scattering of specimen and optical distortion of microscopic system. Therefore, deconvolution becomes a more useful and a crucial approach to restore the original details of neuronal structure in fluorescence images. Since Richardson-Lucy deconvolution algorithm is appropriate for Poisson process of microscopy but sensitive to noise, we propose a scheme that it pre-filters noise via Perona-Malik nonlinear anisotropic diffusion before performing regularized Richardson-Lucy deconvolution algorithm. In contrast to other restoration approaches, the preliminary denoising of Perona-Malik diffusion model provides a better trade-off between noise reduction and edge preservation, and helps to following regularized Richardson-Lucy deconvolution procedure. Experimental results have shown that proposed scheme is effective and robust for restoring noisy two-photon fluorescence images.

Citation Download Citation

Hongmin Zhang, Zhitao Zhang, Qingming Luo, and Shaoqun Zeng "Richardson-Lucy deconvolution for two-photon fluorescence images via non-linear diffusion equation pre-filtering", Proc. SPIE 6436, Complex Dynamics and Fluctuations in Biomedical Photonics IV, 64360P (7 February 2007); https://doi.org/10.1117/12.701750

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available