UV LED fiber optic detection system for DNA and protein

Mathias Belz; Felix A. Klein; Heidi Habhegger

doi:10.1117/12.717552

15 February 2007 UV LED fiber optic detection system for DNA and protein

Mathias Belz, Felix A. Klein, Heidi Habhegger

Proceedings Volume 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII; 64330H (2007) https://doi.org/10.1117/12.717552
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Concentrations of DNA and proteins are traditionally detected at 260/280nm using laboratory spectrophotometers. Recently, AlGaN/GaN ultraviolet Light Emitting Diodes (LED) became available in the 250 nm to 350 nm wavelength region. An inexpensive fiber optic detection system based on these UV LEDs and photodiodes has been developed. It allows concentration measurements of such popular biochemistry samples. Measurement stability and noise will be discussed. The performance of the system in comparison to a standard spectrophotometer will be evaluated. In particular, the effect of decreasing the spectral resolution from usually used 2-3 nm to 10-20 nm Full Width Half Maximum (FWHM) is simulated and experimentally confirmed.

Citation Download Citation

Mathias Belz, Felix A. Klein, and Heidi Habhegger "UV LED fiber optic detection system for DNA and protein", Proc. SPIE 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII, 64330H (15 February 2007); https://doi.org/10.1117/12.717552

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