Europium tetracycline biosensor for the determination of cholesterol

Lilia Coronato Courrol; Flávia Rodrigues de Oliveira Silva; Ricardo Elgul Samad; Ronaldo Domingues Mansano; Nilson Dias Vieira Jr.

doi:10.1117/12.697956

6 February 2007 Europium tetracycline biosensor for the determination of cholesterol

Lilia Coronato Courrol, Flávia Rodrigues de Oliveira Silva, Ricardo Elgul Samad, Ronaldo Domingues Mansano, Nilson Dias Vieira Jr.

Author Affiliations +

Proceedings Volume 6430, Advanced Biomedical and Clinical Diagnostic Systems V; 643017 (2007) https://doi.org/10.1117/12.697956
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Development of cholesterol biosensors is of great importance in clinical analysis because the concentration of cholesterol in blood is a fundamental parameter for the prevention and diagnosis of a number of clinical disorders such as heart disease, hypertension and arteriosclerosis. In general, determination of cholesterol is based on spectrophotometry; but this method involves complicated procedures and the cost is high because expensive enzyme must be used in each assay. We report here the observation, for the first time, of the enhancement of Europium-Tetracycline complex emission in cholesterol solutions. This enhancement was initially observed with the addition of the enzyme cholesterol oxidase, which produces H₂O₂, the agent driver of the Europium tetracycline complex, to the solution. However, it was found that the enzyme is not needed to enhance the luminescence. A calibration curve was determined, resulting in an easy-handling immobilization method with a cheap stable material. This method shows that the complex can be used as a sensor to determine cholesterol in biological systems with good selectivity, fast response, miniature size, and reproducible results.

Citation Download Citation

Lilia Coronato Courrol, Flávia Rodrigues de Oliveira Silva, Ricardo Elgul Samad, Ronaldo Domingues Mansano, and Nilson Dias Vieira Jr. "Europium tetracycline biosensor for the determination of cholesterol", Proc. SPIE 6430, Advanced Biomedical and Clinical Diagnostic Systems V, 643017 (6 February 2007); https://doi.org/10.1117/12.697956

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