Correlation between cell viability and cumulative singlet oxygen luminescence from protoporphyrin IX in varying subcellular localizations

Buhong Li; Mark T. Jarvi; Eduardo H. Moriyama; Brian C. Wilson

doi:10.1117/12.700674

27 February 2007 Correlation between cell viability and cumulative singlet oxygen luminescence from protoporphyrin IX in varying subcellular localizations

Buhong Li, Mark T. Jarvi, Eduardo H. Moriyama, Brian C. Wilson

Author Affiliations +

Proceedings Volume 6427, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI; 642707 (2007) https://doi.org/10.1117/12.700674
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Photodynamic therapy (PDT) can be targeted toward different subcellular localizations and it is widely believed different subcellular targets vary in their sensitivity to photobiological damage. In this study, PDT-generated near-infrared singlet oxygen (¹O₂) luminescence was measured along with cell viability under two different incubation protocols: 5- aminolevulinic acid (ALA) endogenously-induced protoporphyrin IX (PpIX) and exogenous PpIX, at different incubation times. Confocal fluorescence microscopy indicated that ALA-induced PpIX (2 h) localized in the mitochondria, whereas exogenous PpIX (1 h) mainly localized to the plasma membrane. Cell viability was determined at several time points during PDT treatments using colony-forming assays, and the surviving fraction correlated well with cumulative ¹O₂ luminescence counts under both incubation protocols. Preliminary results indicate the plasma membrane is less sensitive to PDT-generated ¹O₂ than the mitochondria.

Citation Download Citation

Buhong Li, Mark T. Jarvi, Eduardo H. Moriyama, and Brian C. Wilson "Correlation between cell viability and cumulative singlet oxygen luminescence from protoporphyrin IX in varying subcellular localizations", Proc. SPIE 6427, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI, 642707 (27 February 2007); https://doi.org/10.1117/12.700674

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