Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector

X. Michalet; O.H. W. Siegmund; J. V. Vallerga; P. Jelinsky; F. Pinaud; J. E. Millaud; S. Weiss

doi:10.1117/12.686429

25 October 2006 Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector

X. Michalet, O.H. W. Siegmund, J. V. Vallerga, P. Jelinsky, F. Pinaud, J. E. Millaud, S. Weiss

Proceedings Volume 6372, Advanced Photon Counting Techniques; 63720E (2006) https://doi.org/10.1117/12.686429
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

We have recently developed a wide-field photon-counting detector (the H33D detector) having high-temporal and highspatial resolutions and capable of recording up to 500,000 photons per sec. Its temporal performance has been previously characterized using solutions of fluorescent materials with different lifetimes, and its spatial resolution using sub-diffraction objects (beads and quantum dots). Here we show its application to fluorescence lifetime imaging of live cells and compare its performance to a scanning confocal TCSPC approach. With the expected improvements in photocathode sensitivity and increase in detector throughput, this technology appears as a promising alternative to the current lifetime imaging solutions.

Citation Download Citation

X. Michalet, O.H. W. Siegmund, J. V. Vallerga, P. Jelinsky, F. Pinaud, J. E. Millaud, and S. Weiss "Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector", Proc. SPIE 6372, Advanced Photon Counting Techniques, 63720E (25 October 2006); https://doi.org/10.1117/12.686429

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