Monitoring cellular mechanosensing using time-correlated single photon counting

Tristan Tabouillot; Ramachandra Gullapalli; Peter J. Butler

doi:10.1117/12.686275

3 November 2006 Monitoring cellular mechanosensing using time-correlated single photon counting

Tristan Tabouillot, Ramachandra Gullapalli, Peter J. Butler

Proceedings Volume 6372, Advanced Photon Counting Techniques; 63720D (2006) https://doi.org/10.1117/12.686275
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

Endothelial cells (ECs) convert mechanical stimuli into chemical signaling pathways to regulate their functions and properties. It is hypothesized that perturbation of cellular structures by force is accompanied by changes in molecular dynamics. In order to address these fundamental issues in mechanosensation and transduction, we have developed a hybrid multimodal microscopy - time-correlated single photon counting (TCSPC) spectroscopy system intended to determine time- and position dependent mechanically-induced changes in the dynamics of molecules in live cells as determined from fluorescence lifetimes and autocorrelation analysis (fluorescence correlation spectroscopy). Colocalization of cell-structures and mechanically-induced changes in molecular dynamics can be done in post-processing by comparing TCSPC data with 3-D models generated from total internal reflection fluorescence (TIRF), differential interference contrast (DIC), epifluorescence, and deconvolution. We present control experiments in which the precise location of the apical cell membrane with respect to a confocal probe is assessed using information obtainable only from TCSPC. Such positional accuracy of TCSPC measurements is essential to understanding the role of the membrane in mechanotransduction. We predict that TCSPC will become a useful method to obtain high temporal and spatial resolution information on localized mechanical phenomena in living endothelial cells. Such insight into mechanotransduction phenomenon may uncover the origins of mechanically-related diseases such as atherosclerosis.

Citation Download Citation

Tristan Tabouillot, Ramachandra Gullapalli, and Peter J. Butler "Monitoring cellular mechanosensing using time-correlated single photon counting", Proc. SPIE 6372, Advanced Photon Counting Techniques, 63720D (3 November 2006); https://doi.org/10.1117/12.686275

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