New fluorescent chemosensors for magnesium ions in living cells

L. Prodi; L. S. Dolci; M. Montalti; N. Zaccheroni; G. Farruggia; G. Andreani; E. Carpenè; S. Iotti; P. B. Savage; V. Trapani; P. Sale; F. I. Wolf

doi:10.1117/12.645783

14 February 2006 New fluorescent chemosensors for magnesium ions in living cells

L. Prodi, L. S. Dolci, M. Montalti, N. Zaccheroni, G. Farruggia, G. Andreani, E. Carpenè, S. Iotti, P. B. Savage, V. Trapani, P. Sale, F. I. Wolf

Author Affiliations +

Proceedings Volume 6097, Optical Molecular Probes for Biomedical Applications; 60970C (2006) https://doi.org/10.1117/12.645783
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Although magnesium ions play a key role in many fundamental biological processes, information about its intracellular regulation is still scarce, due to the lack of appropriate detection methods. Here, we report the spectroscopic characterization of two diaza-18-crown-6 hydroxyquinoline derivatives (DCHQ) and we propose their application for the determination of total Mg²⁺ concentration and in confocal imaging as effective Mg²⁺ indicators. DCHQ derivatives 1 and 2 bind Mg²⁺ with much higher affinity than other available probes (Kd = 44 and 73 mM, respectively) with a concomitant strong fluorescence increase. On the other hand, the fluorescence intensity is not significantly affected by other divalent cations, most importantly Ca²⁺, or by pH changes within the physiological range. Evidence is provided on the use of fluorometric data to derive totalcellular Mg²⁺content, which is in agreement with atomic absorption data. Furthermore, we show that DCHQ compounds can be effectively employed to map intracellular ion distribution and movements in live cells by confocal microscopy. These findings suggest that DCHQ derivatives may serve as new probes for the study of Mg²⁺ regulation, allowing sensitive and straightforward detection of both static and dynamic signals.

Citation Download Citation

L. Prodi, L. S. Dolci, M. Montalti, N. Zaccheroni, G. Farruggia, G. Andreani, E. Carpenè, S. Iotti, P. B. Savage, V. Trapani, P. Sale, and F. I. Wolf "New fluorescent chemosensors for magnesium ions in living cells", Proc. SPIE 6097, Optical Molecular Probes for Biomedical Applications, 60970C (14 February 2006); https://doi.org/10.1117/12.645783

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available