Techniques for moveable traps: the influence of aberration in optical tweezers

Erik Fällman; Magnus Andersson; Ove Axner

doi:10.1117/12.642206

22 February 2006 Techniques for moveable traps: the influence of aberration in optical tweezers

Erik Fällman, Magnus Andersson, Ove Axner

Proceedings Volume 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV; 60881E (2006) https://doi.org/10.1117/12.642206
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

The rapid development of the optical tweezers technique has broadened the applicability of the technique from physics to biology. Although the construction of an optical tweezers system only requires basic skills in optics, the realization of an optical tweezers set up is not always as easy as it seems. A number of designs for moveable traps have been presented in the literature. It is not clear to the readers, however, how the movability of the trap affects its quality. We have therefore scrutinized and compared the most commonly used techniques for steering of an optical trap in terms of the aberrations they introduce. The study shows that a moveable trap based on the movement of a lens introduces significantly more aberration than the systems based on fiber optics or the tilting of a mirror.

Citation Download Citation

Erik Fällman, Magnus Andersson, and Ove Axner "Techniques for moveable traps: the influence of aberration in optical tweezers", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 60881E (22 February 2006); https://doi.org/10.1117/12.642206

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